ABSTRACT
Aim To investigate the anticancer activity of DY386 in a variety of cancer cells and the molecular mechanism of DY386 on cell cycle arrest in DU145 cells. Methods MTT assay was used to determine the inhibitory effect of DY386 on the growth of tumor cells including prostate tumor cell lines DU145, PC-3 ; glioblastoma cell line U87, non-small cell lung cancer cell lines H1975, HCC827, breast cancer cell line MCF-7, hepatoma cell line Hep3B and colon carcinoma cell line SW620. Colony formation assay and BrdU staining assay were performed to check the suppressive activity of DY386 to DU145 cell proliferation. Flow cytometry was conducted to evaluate the cell cycle distribution of DU145. Western blot was performed to detect the pro-tein level of cyclinBl, cyclinEi, CDK2, p27. Results DY386 inhibited cell growth in previous described tumor cell lines, especially in DU145 cells with half maximal inhibitory concentration (IC) value of 11.86 (imol • Flow cytometry analysis indicated that DY386 induced S phase arrest in DU145 cells. Furthermore, Western blot results revealed that DY386 decreased the expression of cyclinEi and cyclinBl and increased the expression of p27 in a dose-dependent manner. Conclusions DY386 reveals certain antitumor effect, and its mechanism may be related to inhibiting cell proliferation by blocking cell cycle.
ABSTRACT
Objective To analyze the relationship between monthly average air temperature and monthly mortality rate among the elderly.Methods The death cause surveillance data between 2011 and 2013 of Yongkang City were used,and the monthly air temperature data was from Statistical Yearbooks published by Bureau of Statistics,and the population data was from the Yongkang public security bureau during this period.The scatter plot of the monthly average temperature and the mortality rate of the elderly were made.According to different causes of death,a linear regression analysis were used to analyze the relationship between the two variables.(The monthly mortality rate was the dependent variable and the monthly average temperature was the independent variable).Results There were negative correlation between the monthly air temperature and the mortality rate caused by circulatory disease(r =-0.884,P <0.001).There were negative correlation between the monthly air temperature and the mortality rate caused by respiratory disease.When the monthly air temperature was ≤15 ℃,and regression mode was Y = -6.399 X +186.813(Adjusted R2 =0.247).No obvious relationship between the monthly air temperature and the mortality rate caused by malignant tumor was observed(R2linear =0.006).Conclusion There is a certain relationship between monthly average air temperature and monthly mortality rate among the elderly. Health -care and nursing of the elderly should be strengthened when the temperature is low,especially for those aged over 80 years.